QuantumScale Single Cell RNA
Ultra-High Throughput Analysis of 2.8 Million Mouse Brain Nuclei with Streamlined Multiplexing
Key highlights:
- Massive scale: Profiled 2.8 million mouse brain nuclei from 768 samples using the flexible, massively scalable QuantumScale Single Cell RNA workflow.
- Streamlined Multiplexing: Leveraging a streamlined, oligo-based multiplexing strategy (ScalePlex), we fixed and pooled 768 samples in just 1 day. Single Cell RNA workflow.
- Robust Cell Annotation: Cell annotation using a global, context-free cellular deconvolution model from Panacent Bio enabled robust cell type identification.
- Optimized Sequencing: Comparison of different sequencing depths demonstrated recovery of similar cell type proportions with increased gene and transcript sensitivity.
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